Dr. Lee has a background in cell biology (Tufts) and trained in internal medicine (University of Pennsylvania). After joining Dr. George Church's laboratory (Harvard), Dr. Lee developed fluorescent in situ sequencing (FISSEQ), in which positional information of transcriptome sequencing reads is retained in single cells in situ. This work was started to interrogate the spatial mosaicism of allele-specific gene expression in the brain with the Allen Institute. As an independent investigator at Cold Spring Harbor Laboratory, the Lee lab is developing targeted RNA-seq methods in situ and setting up the infrastructure needed for high-resolution three-dimensional imaging across the whole tissue. The Lee lab is also developing genetic reporters for multiplexed spatial imaging of single cell lineages using FISSEQ. The current focus of the Lee lab is on human brain gliomas to find RNA biomarkers of metabolic deregulation associated with IDH mutation with Dr. Tim Chan (MSKCC). His lab is also working with Dr. Tony Zador (CSHL) and Dr. Church (Harvard) on developing tools for studying the brain connectivity. The long-term goal is to understand the structural mosaicism of complex genetic traits in neural development, function, and disease.