International Meeting on Optical Biosensors

In memory of Roger Y. Tsien on the 10th year anniversary of his Nobel prize
15-17 November 2018, Ghent, Belgium
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Monday, 5 November, 2018
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International Meeting on Optical Biosensors
In memory of Roger Y. Tsien on the 10th year anniversary of his Nobel prize

Organized by CNRS, ImaBio and IRC

Biological processes show extreme complexity orchestrated both in space and time. To face this complexity, it has been necessary, as pointed out by R.Y. Tsien in his lecture "Unlocking Cell Secrets with Light Beams and Molecular Spies" (Heineken Prize for Biochemistry and Biophysics, 2002), to develop fluorescent spies in order to analyze, quantify in real time and decrypt very sophisticated cellular mechanisms. The recent explosion of fluorescent biosensors development opens a new field of investigation by allowing the analysis of extremely specific biological events at the level of cellular organelles, individual cells, tissues and even entire living organisms in physiological and pathological conditions. The development and the use of these tools require solving a number of conceptual, experimental and technological problems concerning both the biosensors themselves and the tools to follow them. Equally important, a range of new developments including optogenetic and optochemical tools allow systematic perturbation of biochemical processes while they are read out by optical biosensors.

The purpose of this conference is to gather and consolidate the expertise in the field of biosensors and to promote the reflection and the exchange of information so as to accelerate the development of these new dynamic imaging approaches in all areas of biology and biomedical sciences.

Poster information:
Format: A0 (841 x 1189 mm / 33.1 x 46.8 in), portrait orientation

Confirmed speakers

Robert Campbell

University of Alberta, CA

Jin Zhang

University of California, San Diego, CA, US

Carsten Schultz

EMBL, Heidelberg, Germany; OSHU, OR, US

Anders Tengholm

Uppsala University, SE

John Albeck

University of California, Davis, CA, US

Franck Riquet

IRC, VIB-UGent, BE

Alessandro Esposito

MRC, University of Cambridge, UK

Marie Erard

LCP, CNRS UMR 8000, Orsay, FR

Isabelle Limon

IBPS, UPMC-CNRS, Paris, FR

Joachim Goedhart

Universiteit van Amsterdam, NL

Kees Jalink

NKI, Amsterdam, NL

Marc Tramier

IGDR, Rennes, FR

May Morris

IBMM, CNRS, Montpellier, FR

Oliver Griesbeck

MPIN, Martinsried, DE

Peter Dedecker

KU Leuven, Leuven, BE

Pierre Vincent

BAA, Sorbonne Université, Paris, FR

Theodorus Gadella

Universiteit van Amsterdam, NL

Wolf Frommer

Heinrich Heine University Düsseldorf, DE

Day 1 - 15 November 2018

Thursday, 15 November, 2018
11:30 - 12:00
Registration & Welcome Coffee
12:00 - 13:00
Lunch
13:00 - 13:10
Opening remarks reception

The discovery of GFP and a tribute to the contributions of R.Y. Tsien

13:10 - 13:50
Conference lecture
Expanding the palette of genetically-encoded indicators for multi-parameter imaging and manipulation
Robert Campbell
University of Alberta, CA
Academic speaker
13:50 - 17:40
Plenary session 1: New and Improved Fluorescent Proteins

Chairs: Dr. Oliver Griesbeck and Dr. Fabienne Mérola

This session will feature the novel fluorescent proteins and their specific applications in biosensor imaging.

13:50 - 14:20
Enhanced monomeric chromoproteins and red fluorescent proteins for FRET imaging
Theodorus Gadella
Universiteit van Amsterdam, NL
Academic speaker
14:20 - 14:50
Fluorescent proteins as optical reporters for the structural and quantitative characterization of protein-protein interactions in live cell
Marie Erard
LCP, CNRS UMR 8000, Orsay, FR
Academic speaker
14:50 - 15:05
Unexpected events in the functioning of three different fluorescent proteins
Antoine Royant
Institut de Biologie Structurale, FR
15:05 - 15:20
In vivo manipulation of signal transduction using red/infrared light in C. elegans
Shigekazu Oda
National Institute for Basic Biology, JP
15:20 - 15:40
Flash talks

Tou Cheu Xiong: Iron live imaging in Arabidopsis thaliana,
Giulia Bertolin: Optimization of fluorophores and analysis approaches to ameliorate a FRET biosensor of the mitotic kinase AURKA,
Oleg Dyachok: Zn2+-SIGNALING IN INSULIN-SECRETING BETA-CELLS,
Timothy Dore: Photoactivatable dopamine to explore the function of dopaminergic neurons and circuits.

15:40 - 16:10
Coffee break
16:10 - 16:40
New labelling strategies for genetically-encoded biosensors
Peter Dedecker
KU Leuven, Leuven, BE
Academic speaker
16:40 - 17:10
Imaging of G-protein signaling with genetically encoded fluorescent probes
Joachim Goedhart
Universiteit van Amsterdam, NL
Academic speaker
17:10 - 17:40
Tools to Image Single Molecules to Human Disease
Erik Rodriguez
The George Washington University, US
17:40 - 18:10
Coffee break
18:10 - 19:40
Plenary session 2: Functional screening and drug discovery

Chairs: Dr. May Morris and Dr. Alessandro Esposito

In this session we will hear about high-content screening applications and discuss the newest developments aimed at marrying FRET- and FLIM imaging in this context.

18:10 - 18:40
Optimizing FLIM-FRET for fast screening- and signaling applications
Kees Jalink
NKI, Amsterdam, NL
Academic speaker
18:40 - 19:10
Optical biosensors for drug discovery - from conventional kinase inhibitors to allosteric drugs
May Morris
IBMM, CNRS, Montpellier, FR
Academic speaker
19:10 - 19:25
Cathepsin G activity reporters detect chronic lung inflammation by microscopy and flow cytometry
Matteo Guerra
EMBL, DE
19:25 - 19:40
Quantitative analysis of the in vivo performance of fluorescent proteins in yeast
Dennis Botman
Vrije Universiteit Amsterdam, NL
19:40 - 21:10
Reception & Poster Session

Organizing committee:

Peter Dedecker - KU Leuven, Leuven, BE;
Oliver Griesbeck - MPIN, Martinsried, DE;
Kees Jalink - NKI, Amsterdam, NL;
Saskia Lippens - VIB BioImaging Core and IRC, VIB-UGent, Ghent, BE;
Juan Llopis - CRIB, UCLM, Albacete, ES;
May Morris - IBMM, CNRS, Montpellier, FR;
Clotilde Randriamampita - Institut Cochin, CNRS, INSERM, Paris, FR;
Franck Riquet - Head of the Committee; IRC, VIB-UGent, Ghent, BE; Université de Lille, Lille, FR;
Peter Vandenabeele - IRC, VIB-UGent, Ghent, BE;
Pierre Vincent - BAA, Sorbonne Université, Paris, FR.

Day 2 - 16 November 2018

Friday, 16 November, 2018
08:30 - 09:00
Coffee
09:00 - 09:40
Conference lecture
Illuminating the Biochemical Activity Architecture of the Cell
Jin Zhang
University of California, San Diego, CA, US
Academic speaker
09:40 - 13:10
Plenary session 3: Monitoring the Dynamics of Biological Events in Living Cells

Chairs: Dr. Clotilde Randriamampita and Dr. Pierre Vincent

Imaging biosensors in a physiologically-relevant preparation can be challenging. This session will present a selection of biological discoveries in which biosensor imaging was instrumental.

09:40 - 10:10
Dynamic control of insulin secretion - a real-time view beneath the beta-cell plasma membrane
Anders Tengholm
Uppsala University, SE
Academic speaker
10:10 - 10:40
Studying the spatio-temporal regulation of unperturbed Life and Death cellular processes: Easier said than done!
Franck Riquet
IRC, VIB-UGent, BE
Academic speaker
10:40 - 11:10
Integration of growth factor signaling and glucose metabolism in single cells
John Albeck
University of California, Davis, CA, US
Academic speaker
11:10 - 11:40
Coffee break
11:40 - 12:00
Novel short isoforms of adenylyl cyclase as negative regulators of cAMP production
Isabelle Limon
IBPS, UPMC-CNRS, Paris, FR
Academic speaker
12:00 - 12:30
Dopamine, phosphodiesterases and cyclic nucleotide dynamics in the striatum
Pierre Vincent
BAA, Sorbonne Université, Paris, FR
12:30 - 12:45
Dopamine D1 and metabotropic glutamate mGlu5 receptors form heteromers whose activation favors Ca2+ signaling pathway
Julie Perroy
IGF Montpellier, FR
12:45 - 13:00
Real-time measurements of cGMP in cardiac and hippocampal cells
Michael Russwurm
Ruhr-University Bochum, DE
13:00 - 14:30
Lunch
14:30 - 18:00
Plenary session 4: Probe development, optimization and performance

Chairs: Pr. Franck Riquet and Pr. Juan Llopis

Better biosensors allow for more applications. This session will focus on how biosensor performances can be improved, in terms of signal, photostability, vectorization etc...

14:30 - 15:00
A Screening Platform for Optimizing Fluorophores and Biosensors
Oliver Griesbeck
MPIN, Martinsried, DE
Academic speaker
15:00 - 15:30
Live-cell FRET imaging reveals propagating waves of ERK activation and its function in collective cell migration
Kazuhiro Aoki
National Institute for Basic Biology, JP
15:30 - 15:50
Flash talks

R Harkes: A gated solid-state camera for high throughput frequency domain FLIM measurements of cAMP dynamics,
Ana Clara Fernandes (Nikon): Bring high resolution, high content imaging to life,
Fabien Miart: Functional in vivo imaging of pH and Ca2+ dynamics in Arabidopsis thaliana growing seedlings using biosensors and the VIP-box,
Jochen Sieber (Leica): SP8 FALCON – a new approach for lifetime imaging.

15:50 - 16:30
Coffee break
16:30 - 17:00
New fluorescent probes for imaging disease-relevant events
Carsten Schultz
EMBL, Heidelberg, Germany; OSHU, OR, US
Academic speaker
17:00 - 17:30
Monitoring real-time HIV-1 virion fusion and downstream metabolic consequences with FRET-based biosensors
Sergi Padilla-Parra
University of Oxford, GB
17:30 - 17:45
Coordinated Histone Modifications and Chromatin Reorganization Visualized in Single Live Cells
Chiwei Man
UCSD, US
17:45 - 18:00
Development of an affine and selective uranium biosensor and its use for environmental monitoring
Rym Cherif
CEA, CNRS, Aix-Marseille Université, UMR, FR
18:00 - 20:00
Walking dinner & Poster Session

'Fiers-Schell-Van Montagu' building

Organizing committee:

Peter Dedecker - KU Leuven, Leuven, BE;
Oliver Griesbeck - MPIN, Martinsried, DE;
Kees Jalink - NKI, Amsterdam, NL;
Saskia Lippens - VIB BioImaging Core and IRC, VIB-UGent, Ghent, BE;
Juan Llopis - CRIB, UCLM, Albacete, ES;
May Morris - IBMM, CNRS, Montpellier, FR;
Clotilde Randriamampita - Institut Cochin, CNRS, INSERM, Paris, FR;
Franck Riquet - Head of the Committee; IRC, VIB-UGent, Ghent, BE; Université de Lille, Lille, FR;
Peter Vandenabeele - IRC, VIB-UGent, Ghent, BE;
Pierre Vincent - BAA, Sorbonne Université, Paris, FR.

Day 3 - 17 November 2018

Saturday, 17 November, 2018
08:30 - 09:00
Coffee
09:00 - 09:40
Conference lecture
In vivo biochemistry with sensors for signaling molecules and transporters
Wolf Frommer
Heinrich Heine University Düsseldorf, DE
Academic speaker
09:40 - 13:10
Plenary session 5: Advances in methodologies and instrumentation

Chairs: Dr. Saskia Lippens and Pr. Peter Dedecker

The pros and cons of the various detection instruments will be illustrated and discussed, as well as how the signal can be quantified and deemed relevant of a biological event.

09:40 - 10:10
Multiplex FRET by FLIM: how to reveal a force gradient across talin
Marc Tramier
IGDR, Rennes, FR
Academic speaker
10:10 - 10:40
Live cell biochemistry by light
Alessandro Esposito
MRC, University of Cambridge, UK
Academic speaker
10:40 - 10:55
Advanced spectral FRET approaches for quantitative Imaging reveal importance of receptor oligomerization in serotonergic signalling
Andre Zeug
Hannover Medical School, DE
10:55 - 11:10
Coupling magneto-active substrates with FRET biosensors to decode mechanotransduction
Alain Henri Lombard
Laboratoire Interdisciplinaire de Physique, FR
11:10 - 11:40
Coffee break
11:40 - 11:55
Studying cardiac function with Ca2+ biosensors in zebrafish (Danio rerio) embryos
Jussep Salgado
CRIB and Albacete School of Medicine, University of Castilla-La Mancha, ES
11:55 - 12:10
In vivo optical mapping of cardiac action potentials in zebrafish larvae
Ewa Sieliwonczyk
University of Antwerp, BE
12:10 - 12:40
Visualizing cellular heterogeneity in ERK signaling in patient-derived cancer organoids using an improved, cell-cycle insensitive, FRET sensor
Bas Ponsioen
University Medical Center Utrecht, NL
12:40 - 13:10
In vivo quantification of FRET biosensors using multispectral FLIM-FRET
Sean Warren
Garvan Institute of Medical Research, AU
13:10 - 13:15
Closing remarks
13:15 - 14:30
Lunch

Organizing committee:

Peter Dedecker - KU Leuven, Leuven, BE;
Oliver Griesbeck - MPIN, Martinsried, DE;
Kees Jalink - NKI, Amsterdam, NL;
Saskia Lippens - VIB BioImaging Core and IRC, VIB-UGent, Ghent, BE;
Juan Llopis - CRIB, UCLM, Albacete, ES;
May Morris - IBMM, CNRS, Montpellier, FR;
Clotilde Randriamampita - Institut Cochin, CNRS, INSERM, Paris, FR;
Franck Riquet - Head of the Committee; IRC, VIB-UGent, Ghent, BE; Université de Lille, Lille, FR;
Peter Vandenabeele - IRC, VIB-UGent, Ghent, BE;
Pierre Vincent - BAA, Sorbonne Université, Paris, FR.

Attendees

The full list of attendees will be available on 09-11-2018 until 29-11-2018.
This list will only be available for registered attendees of the event.

Abstracts

The full list of abstracts will be available on 09-11-2018 until 29-11-2018.
Only registered attendees can download abstracts.

Venue

VIB-UGent Center for Inflammation Research
a VIB-UGent Department
'Fiers-Schell-Van Montagu' building
Technologiepark 927
9052 Ghent (Zwijnaarde)
Belgium

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