After a PhD in biophysics in 2001 supervised by Dr M. Coppey-Moisan, Marc Tramier was directly recruited as CNRS Research Engineer in Maïté Coppey ’s lab at Jacques Monod Institute (Paris). In 2010, Marc Tramier moved to Rennes to set up his own team at Institut de Génétique et Développement de Rennes. Marc Tramier is renowned for his expertise in FLIM and particularly its applications in biology to quantify FRET in live samples. His team aims at developing techniques and methodologies in fluorescence microscopy to study dynamics of protein-protein interactions and biochemical activities in live sample. Team approaches are mainly driven by methodological and technological development, its transfer and its applicability in biology to answer relevant new questions.